2018.12

コンピテントセル作成

2018.12.17 16:09

１．種菌をLB plateにストリーク(O/N)…① ２．コロニー２,３個をつつき、数ml(3～5ml)のLBで振盪培養(5時間程度)…② ３．コンピテントセル培養溶液*で振盪培養(18℃、O/N～2日)…③分光光度計で菌の濃度を確かめ、OD600＝0.4～0...

Pygmy Squid Whole-Mount In Situ Hybridization

2018.12.11 09:37

This protocol is largely based on Yu and Holland (2009) with some modification. Some procedures followed Ono et al. (2014) an...